交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 ng/ml - 50 ng/ml
基因名全称
lipopolysaccharide binding protein
文献引用格式
Human LBP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1271)
背景资料
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1271-CAP | 96-well plate precoated with anti- human LBP antibody | 1 |
EK1271-ST | Lyophilized recombinant human LBP standard | 50ng/tube×2 |
EK1271-DA | Biotinylated anti- human LBP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1485 | 4533 | 23562 | 1518 | 4575 | 21374 |
Standard deviation | 66.82 | 271.98 | 1507.96 | 85 | 297.37 | 1432.05 |
CV(%) | 4.5 | 6 | 6.4 | 5.6 | 6.5 | 6.7 |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.096 | 0.147 | 0.218 | 0.358 | 0.591 | 0.913 | 1.407 | 2.090 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)