Bovine CXCL14 ELISA Kit

96T/2480

BMAC; bolekine; BRAK; C X C motif chemokine 14; Chemokine BRAK; CXCL14; Kec; KS1; MIP 2g; MIP2G; NJAC; PSEC0212; SCYB14; Small inducible cytokine B14

Expression system for standard: E.coli; Immunogen sequence: S35-E111

There is no detectable cross-reactivity with other relevant proteins.

<60 pg/ml

125pg/ml-4000pg/ml

CXCL14

C-X-C motif chemokine 14

O95715

Bovine CXCL14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1285-BV)

Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

20-25分钟

Catalog number	Description	Quantity
EK1285-BV-CAP	Anti-Bovine CXCL14 Pre-coated 96-well strip microplate	1
EK1285-BV-ST	Bovine CXCL14 Standard	10ng/tube×2
EK1285-BV-DA	Bovine CXCL14 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	987	1638	3285	1387	2694	3525
Standard Deviation	35.5	68.8	187.2	56.87	156	224.2
CV (%)	3.6	4.2	5.7	4.1	5.8	6.4

Concentration(pg/ml)	0	125	250	500	1000	2000	4000
O.D.	0.016	0.063	0.118	0.229	0.406	0.790	1.817

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)