产品名称
Bovine CXCL14 ELISA Kit
指标别名
BMAC; bolekine; BRAK; C X C motif chemokine 14; Chemokine BRAK; CXCL14; Kec; KS1; MIP 2g; MIP2G; NJAC; PSEC0212; SCYB14; Small inducible cytokine B14
免疫原
Expression system for standard: E.coli; Immunogen sequence: S35-E111
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-X-C motif chemokine 14
文献引用格式
Bovine CXCL14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1285-BV)
背景资料
Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.
试剂盒内容
Catalog number | Description | Quantity |
EK1285-BV-CAP | Anti-Bovine CXCL14 Pre-coated 96-well strip microplate | 1 |
EK1285-BV-ST | Bovine CXCL14 Standard | 10ng/tube×2 |
EK1285-BV-DA | Bovine CXCL14 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 987 | 1638 | 3285 | 1387 | 2694 | 3525 |
Standard Deviation | 35.5 | 68.8 | 187.2 | 56.87 | 156 | 224.2 |
CV (%) | 3.6 | 4.2 | 5.7 | 4.1 | 5.8 | 6.4 |
典型数据示例
Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.016 | 0.063 | 0.118 | 0.229 | 0.406 | 0.790 | 1.817 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)