Catalog number | Description | Quantity |
---|---|---|
EK1312-CAP | 96-well plate precoated with anti- human CD32 antibody | 1 |
EK1312-ST | Lyophilized recombinant human CD32 standard | 10ng/tube×2 |
EK1312-DA | Biotinylated anti- human CD32 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 122 | 404 | 1372 | 133 | 365 | 1478 |
Standard deviation | 8.05 | 19.79 | 97.41 | 9.7 | 22.26 | 130.06 |
CV(%) | 6.6 | 4.9 | 7.1 | 7.3 | 6.1 | 8.8 |
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.007 | 0.124 | 0.199 | 0.255 | 0.541 | 0.813 | 1.408 | 2.266 |
Human CD32/FCGR2b/c ELISA Kit standard curve
Human CD32/FCGR2b/c ELISA Kit standard curve