Mouse Neuropilin-2/NRP2 ELISA Kit

96T|2480

neuropilin 2; 1110048P06Rik; N; Np; Np-2; Np2; Npn-2; Npn2; Neuropilin-2; NRP2

Expression system for standard: NS0; Immunogen sequence: Q23-D863

There is no detectable cross-reactivity with other relevant proteins.

156pg/ml-10000pg/ml

NRP2

Neuropilin-2

O35375  

Mouse Neuropilin-2/NRP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1344)

NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Catalog number	Description	Quantity
EK1344-CAP	96-well plate precoated with anti- mouse Neuropilin-2 antibody	1
EK1344-ST	Lyophilized recombinant mouse Neuropilin-2 standard	10ng/tube×2
EK1344-DA	Biotinylated anti- mouse Neuropilin-2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	267	1352	5179	243	1366	4747
Standard deviation	17.62	66.24	346.99	16.52	77.86	394
CV(%)	6.6%	4.9%	6.7%	6.8%	5.7%	8.3%

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.052	0.183	0.142	0.234	0.432	0.723	1.216	1.931

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Cell Lysates

3.5h