产品名称
Human IL-1F7/IL37 ELISA Kit
指标别名
FIL1; FIL1 zeta; FIL1(ZETA); FIL1Z; IL 1 zeta; IL 1F7; IL 1H4; IL 1RP1; IL 1X; IL1F7; IL-1F7; IL1H4; IL1RP1; IL-37; IL 37; Interleukin 1 family member 7; Interleukin 1 homolog 4; Interleukin 1 related protein; Interleukin 1 zeta
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human IL-1F7/IL37 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1363)
背景资料
Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1363-CAP | 96-well plate precoated with anti- human IL-37 antibody | 1 |
EK1363-ST | Lyophilized recombinant human IL-37 standard | 10ng/tube×2 |
EK1363-DA | Biotinylated anti- human IL-37 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 145 | 440 | 36 | 134 | 419 |
Standard deviation | 2.61 | 5.94 | 22.88 | 2.95 | 7.1 | 22.62 |
CV(%) | 7.7 | 4.1 | 5.2 | 8.2 | 5.3 | 5.4 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.004 | 0.069 | 0.135 | 0.230 | 0.495 | 0.905 | 1.721 | 2.550 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)