产品名称
Human Neuregulin-1/NRG1-beta1 ELISA Kit
指标别名
ARIA; GGF; GGF2; Glial growth factor; Heregulin; HGL; HRG; HRG1; HRGA; NDF; Neu differentiation factor; neuregulin 1; NRG1; Pro NRG1; SMDF
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human Neuregulin-1/NRG1-beta1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1378)
背景资料
Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1378-CAP | 96-well plate precoated with anti- human Neuregulin-1 antibody | 1 |
EK1378-ST | Lyophilized recombinant human Neuregulin-1 standard | 10ng/tube×2 |
EK1378-DA | Biotinylated anti- human Neuregulin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 158 | 621 | 1900 | 159 | 607 | 1910 |
Standard deviation | 12.16 | 30.42 | 81.7 | 14.46 | 40.06 | 95.5 |
CV(%) | 7.7 | 4.9 | 4.3 | 9.1 | 6.6 | 5 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.031 | 0.095 | 0.175 | 0.299 | 0.596 | 1.008 | 1.651 | 2.311 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)