Mouse FGF21 ELISA Kit

96T/2480

FGF 21; FGF21; FGF-21; fibroblast growth factor 21

Expression system for standard: E.coli; Immunogen sequence: A29-S210

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

Fgf21

Fibroblast growth factor 21

Q9JJN1  

Mouse FGF21 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1379)

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Catalog number	Description	Quantity
EK1379-CAP	96-well plate precoated with anti- Mouse FGF21 antibody	1
EK1379-ST	Lyophilized recombinant Mouse FGF21 standard	10ng/tube×2
EK1379-DA	Biotinylated anti- Mouse FGF21 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	64	320	918	65	289	938
Standard deviation	2.81	24	52.32	3.12	27.16	70.35
CV(%)	4.4%	7.5%	5.7%	4.8%	9.4%	7.5%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.019	0.093	0.184	0.322	0.562	0.996	1.652	2.395

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h