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Human STC1 ELISA Kit

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EK1404

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human STC1 ELISA Kit
规格/价格
96T|2480
指标别名
stanniocalcin 1; STC; STC 1; STC1
免疫原
NSO, V34-A247
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
STC1
基因名全称
stanniocalcin 1
Uniprot ID
P52823  
文献引用格式
Human STC1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1404)
背景资料
Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1404-CAP96-well plate precoated with anti- human STC1 antibody1
EK1404-STLyophilized recombinant human STC1 standard10ng/tube×2
EK1404-DABiotinylated anti- human STC1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)752791061713061123
Standard deviation3.913.1156.234.0419.2760.64
CV(%)5.24.75.35.76.35.4
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0560.0990.1450.2050.3480.62541.1351.946
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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