产品名称
Human STC1 ELISA Kit
指标别名
stanniocalcin 1; STC; STC 1; STC1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human STC1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1404)
背景资料
Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1404-CAP | 96-well plate precoated with anti- human STC1 antibody | 1 |
| EK1404-ST | Lyophilized recombinant human STC1 standard | 10ng/tube×2 |
| EK1404-DA | Biotinylated anti- human STC1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 75 | 279 | 1061 | 71 | 306 | 1123 |
| Standard deviation | 3.9 | 13.11 | 56.23 | 4.04 | 19.27 | 60.64 |
| CV(%) | 5.2 | 4.7 | 5.3 | 5.7 | 6.3 | 5.4 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.056 | 0.099 | 0.145 | 0.205 | 0.348 | 0.6254 | 1.135 | 1.946 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)