Human Caspase 3/CASP3 ELISA Kit

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EK1425

96T ￥2480
货期：现货

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Human Caspase 3 ELISA Kit standard curve
all(2)
all(2)

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产品简介>

产品名称

Human Caspase 3/CASP3 ELISA Kit

规格/价格

96T/2480

指标别名

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

免疫原

E.coli,S29-D175&A183-H277

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

CASP3

基因名全称

caspase 3

Uniprot ID

P42574

文献引用格式

Human Caspase 3/CASP3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1425)

背景资料

Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK1425-CAP	96-well plate precoated with anti- human Caspase 3 antibody	1
EK1425-ST	Lyophilized recombinant human Caspase 3 standard	10ng/tube×2
EK1425-DA	Biotinylated anti- human Caspase 3 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	63	211	945	69	203	995
Standard deviation	3.27	15.4	55.75	4.2	17.86	62.68
CV(%)	5.2	7.3	5.9	6.1	8.8	6.3

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.020	0.069	0.114	0.228	0.443	0.783	1.395	2.220

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Caspase 3/CASP3 ELISA Kit

EK1425

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(6) All Species Human(3) Rat(3) 清除

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Human Caspase 3/CASP3 ELISA Kit

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