指标别名
BMP 7; BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP 1; OP1; Osteogenic protein 1
免疫原
Expression system for standard: CHO; Immunogen sequence: S292-H430
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
bone morphogenetic protein 7
文献引用格式
Rat BMP7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1444)
背景资料
Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
试剂盒内容
Catalog number | Description | Quantity |
EK1444-CAP | 96-well plate precoated with anti- rat BMP-7 antibody | 1 |
EK1444-ST | Lyophilized recombinant rat BMP-7 standard | 10ng/tube×2 |
EK1444-DA | Biotinylated anti- rat BMP-7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 86 | 190 | 752 | 93 | 186 | 766 |
Standard deviation | 4.38 | 14.44 | 36.09 | 5.76 | 16.55 | 49.79 |
CV(%) | 5.1% | 7.6% | 4.8% | 6.2% | 8.9% | 6.5% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.084 | 0.129 | 0.171 | 0.233 | 0.391 | 0.712 | 1.320 | 2.240 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma ((heparin, EDTA)