Human ADA ELISA Kit

96T/2480

ADA; Adenosine aminohydrolase; adenosine deaminase

sf21, A2-L363

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2000 pg/ml

ADA

adenosine deaminase

P00813  

Human ADA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1446)

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

15-20分钟

Catalog number	Description	Quantity
EK1446-CAP	96-well plate precoated with anti- human ADA antibody	1
EK1446-ST	Lyophilized recombinant human ADA standard	10ng/tube×2
EK1446-DA	Biotinylated anti- human ADA antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	76	257	1026	81	240	1095
Standard deviation	4.63	12.33	41.04	5.91	11.52	53.65
CV(%)	6.1	4.8	4	7.3	4.8	4.9

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.051	0.118	0.196	0.299	0.574	0.995	1.753	2.449

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and pleural fluid

3.5h