产品名称
Human Afamin/AFM ELISA Kit
指标别名
afamin; AFM; ALB2; ALBA; ALF; Alpha Alb; Alpha albumin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Afamin/AFM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1487)
背景资料
Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1487-CAP | 96-well plate precoated with anti- human Afamin antibody | 1 |
| EK1487-ST | Lyophilized recombinant human Afamin standard | 2ng/tube×2 |
| EK1487-DA | Biotinylated anti- human Afamin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 54 | 287 | 838 | 49 | 308 | 783 |
| Standard deviation | 2.75 | 18.94 | 61.17 | 2.84 | 25.87 | 60.29 |
| CV(%) | 5.1 | 6.6 | 7.3 | 5.8 | 8.4 | 7.7 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.068 | 0.093 | 0.121 | 0.193 | 0.354 | 0.715 | 1.559 | 2.457 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Urine