产品名称
Human Afamin/AFM ELISA Kit
指标别名
afamin; AFM; ALB2; ALBA; ALF; Alpha Alb; Alpha albumin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Afamin/AFM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1487)
背景资料
Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1487-CAP | 96-well plate precoated with anti- human Afamin antibody | 1 |
EK1487-ST | Lyophilized recombinant human Afamin standard | 2ng/tube×2 |
EK1487-DA | Biotinylated anti- human Afamin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 54 | 287 | 838 | 49 | 308 | 783 |
Standard deviation | 2.75 | 18.94 | 61.17 | 2.84 | 25.87 | 60.29 |
CV(%) | 5.1 | 6.6 | 7.3 | 5.8 | 8.4 | 7.7 |
典型数据示例
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.030 | 0.149 | 0.251 | 0.480 | 0.751 | 1.261 | 1.806 | 2.089 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Urine