产品名称
Human SERPINA7 ELISA Kit
指标别名
SERPINA7; TBG; Thyroxine-binding globulin; Serpin A7; T4-binding globulin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
serpin family A member 7
文献引用格式
Human SERPINA7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1510)
背景资料
Thyroxine-binding globulin (TBG), also known as SERPINA7, is the major thyroid hormone transport protein in serum. There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. Using DNA blot hybridization, it is detected that there is a single-copy TBG gene located on the long arm of the X chromosome.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1510-CAP | 96-well plate precoated with anti- human Serpin A7 antibody | 1 |
EK1510-ST | Lyophilized recombinant human Serpin A7 standard | 10ng/tube×2 |
EK1510-DA | Biotinylated anti- human Serpin A7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 81 | 314 | 875 | 75 | 294 | 845 |
Standard deviation | 4.94 | 16.64 | 45.5 | 5.47 | 15.87 | 49.01 |
CV(%) | 6.1 | 5.3 | 5.2 | 7.3 | 5.4 | 5.8 |
典型数据示例
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.062 | 0.155 | 0.204 | 0.346 | 0.606 | 0.969 | 1.659 | 2.063 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)