产品名称
Human DEFA1 ELISA Kit
指标别名
DEF1; DEFA1; DEFA1B; DEFA2; defensin; alpha 1; HNP-1; HNP2; HNP 2; HNP-2; HP1; HP 1; HP-1; HP 2; HP-2; HP2; MRS; Neutrophil defensin 1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
文献引用格式
Human DEFA1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1514)
背景资料
Defensin is a humanproteinthat is encoded by theDEFA1gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1514-CAP | 96-well plate precoated with anti- human DEFA1 antibody | 1 |
EK1514-ST | Lyophilized recombinant human DEFA1 standard | 10ng/tubex2 |
EK1514-DA | Biotinylated anti- human DEFA1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 462 | 1682 | 9496 | 635 | 1897 | 9984 |
Standard deviation | 21.71 | 89.15 | 531.78 | 42.55 | 96.75 | 479.23 |
CV(%) | 4.7 | 5.3 | 5.6 | 6.7 | 5.1 | 4.8 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.091 | 0.139 | 0.172 | 0.203 | 0.353 | 0.633 | 1.277 | 2.163 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)