产品名称
Human ALK-1/ACVRL1 ELISA Kit
指标别名
Activin receptor like kinase 1; ACVRL1; ACVRLK1; ALK 1; ALK1; HHT; HHT2; ORW2; SKR3; TSR I
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
46.9 pg/ml - 3000 pg/ml
基因名全称
activin A receptor like type 1
文献引用格式
Human ALK-1/ACVRL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1518)
背景资料
Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1518-CAP | 96-well plate precoated with anti- human ALK-1 antibody | 1 |
EK1518-ST | Lyophilized recombinant human ALK-1 standard | 10ng/tube×2 |
EK1518-DA | Biotinylated anti- human ALK-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 110 | 377 | 1376 | 111 | 366 | 1411 |
Standard deviation | 5.83 | 17.71 | 71.55 | 5.88 | 22.69 | 73.37 |
CV(%) | 5.3 | 4.7 | 5.2 | 5.3 | 6.2 | 5.2 |
典型数据示例
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.114 | 0.152 | 0.194 | 0.269 | 0.465 | 0.785 | 1.342 | 2.049 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)