产品名称
Mouse Artemin/ARTN ELISA Kit
指标别名
Artemin; Enovin; Neublastin; ARTN; EVN; NBN
免疫原
Expression system for standard: E.coli; Immunogen sequence: A112-G224
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Artemin/ARTN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1535)
背景资料
Artemin, also known as enovin or neublastin, is a protein that in humans is encoded by the ARTN gene. This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1535-CAP | 96-well plate precoated with anti- mouse Artemin antibody | 1 |
EK1535-ST | Lyophilized recombinant mouse Artemin standard | 10ng/tube×2 |
EK1535-DA | Biotinylated anti- mouse Artemin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 59 | 286 | 954 | 57 | 312 | 938 |
Standard deviation | 3.3 | 16.3 | 45.79 | 3.36 | 20.9 | 51.59 |
CV(%) | 5.6% | 5.7% | 4.8% | 5.9% | 6.7% | 5.5% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.183 | 0.303 | 0.500 | 0.737 | 1.216 | 1.833 | 2.319 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)