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Rat ALCAM ELISA Kit

说明书

EK1572

  • 96T ¥2480
  • 货期: 现货

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产品名称
Rat ALCAM ELISA Kit
规格/价格
96T/2480
指标别名
ALCAM; CD166; CD166 antigen; MEMD
免疫原
Expression system for standard: NS0; Immunogen sequence: W28-K527
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78pg/ml-5000pg/ml
基因名称
ALCAM
基因名全称
activated leukocyte cell adhesion molecule
Uniprot ID
O35112  
文献引用格式
Rat ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1572)
背景资料
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1572-CAP96-well plate precoated with anti- rat ALCAM antibody1
EK1572-STLyophilized recombinant rat ALCAM standard10ng/tube×2
EK1572-DABiotinylated anti- rat ALCAM antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)12365923751246342509
Standard deviation6.3950.74187.627.9354.52225.81
CV(%)5.2%7.7%7.9%6.4%8.6%9%
典型数据示例
Concentration(pg/ml)078156312625125025005000
O.D.0.0040.0640.1180.2150.4500.7981.5782.317
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

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