指标别名
ALCAM; CD166; CD166 antigen; MEMD
免疫原
Expression system for standard: NS0; Immunogen sequence: W28-K527
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
activated leukocyte cell adhesion molecule
文献引用格式
Rat ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1572)
背景资料
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
试剂盒内容
Catalog number | Description | Quantity |
EK1572-CAP | 96-well plate precoated with anti- rat ALCAM antibody | 1 |
EK1572-ST | Lyophilized recombinant rat ALCAM standard | 10ng/tube×2 |
EK1572-DA | Biotinylated anti- rat ALCAM antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 123 | 659 | 2375 | 124 | 634 | 2509 |
Standard deviation | 6.39 | 50.74 | 187.62 | 7.93 | 54.52 | 225.81 |
CV(%) | 5.2% | 7.7% | 7.9% | 6.4% | 8.6% | 9% |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.004 | 0.064 | 0.118 | 0.215 | 0.450 | 0.798 | 1.578 | 2.317 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)