指标别名
A FABP; AFABP; ALBP; aP2; FABP4; Fatty acid binding protein 4
免疫原
Expression system for standard: E.coli; Immunogen sequence: M1-A132
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
fatty acid binding protein 4
文献引用格式
Rat FABP4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1573)
背景资料
FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1573-CAP | 96-well plate precoated with anti- rat FABP4 antibody | 1 |
| EK1573-ST | Lyophilized recombinant rat FABP4 standard | 10ng/tube×2 |
| EK1573-DA | Biotinylated anti- rat FABP4 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 290 | 1382 | 5455 | 295 | 1277 | 5547 |
| Standard deviation | 13.05 | 82.92 | 223.65 | 14.45 | 80.45 | 266.25 |
| CV(%) | 4.5% | 6% | 4.1% | 4.9% | 6.3% | 4.8% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.072 | 0.109 | 0.149 | 0.21 | 0.377 | 0.656 | 1.124 | 1.963 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Cell Lysates