指标别名
Fibroblast growth factor 23; FGF-23; Fgf23; FGF23
免疫原
Expression system for standard: E.coli; Immunogen sequence: Y25-V251
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
fibroblast growth factor 23
文献引用格式
Rat FGF23 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1591)
背景资料
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
试剂盒内容
Catalog number | Description | Quantity |
EK1591-CAP | 96-well plate precoated with anti- rat FGF23 antibody | 1 |
EK1591-ST | Lyophilized recombinant rat FGF23 standard | 10ng/tubex2 |
EK1591-DA | Biotinylated anti- rat FGF23 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 55 | 244 | 925 | 59 | 248 | 978 |
Standard deviation | 2.81 | 13.18 | 39.88 | 2.9 | 16.6 | 77.3 |
CV(%) | 5.1% | 5.4% | 4.3% | 4.9% | 6.7% | 7.9% |
典型数据示例
Concentration(pg/ml) | 0.0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.108 | 0.236 | 0.303 | 0.655 | 0.762 | 1.201 | 1.735 | 2.091 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)