产品名称
Mouse FABP2 ELISA Kit
指标别名
FABP2; FABPI; Fatty acid binding protein 2; I FABP
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-E132
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
fatty acid binding protein 2, intestinal
文献引用格式
Mouse FABP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1622)
背景资料
Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1622-CAP | 96-well plate precoated with anti- mouse FABP2 antibody | 1 |
EK1622-ST | Lyophilized recombinant mouse FABP2 standard | 10ng/tube×2 |
EK1622-DA | Biotinylated anti- mouse FABP2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 75 | 265 | 861 | 69 | 288 | 910 |
Standard deviation | 5.92 | 13.78 | 38.74 | 5.93 | 17.56 | 56.42 |
CV(%) | 7.9% | 5.2% | 4.5% | 8.6% | 6.1% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.051 | 0.117 | 0.194 | 0.306 | 0.525 | 0.899 | 1.423 | 2.182 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)