产品名称
Human Pro-Collagen Iα1/COL1A1 ELISA Kit
指标别名
Alpha 1 type I collagen; COL1A1; Collagen alpha 1(I) chain; collagen; type I; alpha 1; OI4
免疫原
CHO,Q23-K277 + G1094-L1464
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
collagen type I alpha 1 chain
文献引用格式
Human Collagen Type I/COL1A1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1692)
背景资料
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1692-CAP | 96-well plate precoated with anti- human COL1A1 antibody | 1 |
EK1692-ST | Lyophilized recombinant human COL1A1 standard | 10ng/tubex2 |
EK1692-DA | Biotinylated anti- human COL1A1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 105 | 360 | 1669 | 78 | 251 | 1684 |
Standard deviation | 3.03 | 19.01 | 123.34 | 5.65 | 11.55 | 105.59 |
CV(%) | 2.9 | 5.4 | 7.4 | 7.2 | 4.6 | 6.3 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.026 | 0.09 | 0.119 | 0.215 | 0.374 | 0.716 | 1.249 | 2.052 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)