产品名称
Rat Agrin/AGRN ELISA Kit
免疫原
Expression system for standard: NS0; Immunogen sequence: A1153-P1959
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat Agrin/AGRN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1699)
背景资料
Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
试剂盒内容
Catalog number | Description | Quantity |
EK1699-CAP | 96-well plate precoated with anti- rat Agrin antibody | 1 |
EK1699-ST | Lyophilized recombinant rat Agrin standard | 10ng/tubex2 |
EK1699-DA | Biotinylated anti- rat Agrin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 57 | 219 | 767 | 60 | 223 | 776 |
Standard deviation | 2.39 | 10.73 | 39.1 | 3.36 | 14.27 | 50.1 |
CV(%) | 4.2% | 4.9% | 5.1% | 5.6% | 6.4% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.079 | 0.156 | 0.211 | 0.336 | 0.565 | 0.991 | 1.741 | 2.376 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)