Human TWEAKR/TNFRSF12A ELISA Kit

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EK1753

96T ￥2800
货期：现货

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Human TNFRSF12A/TWEAKR ELISA Kit standard curve
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产品简介>

产品名称

Human TWEAKR/TNFRSF12A ELISA Kit

规格/价格

96T/2800

指标别名

TNF receptor superfamily member 12A; CD266; FN14; TWEAKR; TNFRSF12A

免疫原

E.coli, E28-P80

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

TNFRSF12A

基因名全称

TNF receptor superfamily member 12A

Uniprot ID

Q9NP84

文献引用格式

Human TWEAKR/TNFRSF12A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1753)

背景资料

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK1753-CAP	96-well plate precoated with anti- human TNFRSF12A/TWEAKR antibody	1
EK1753-ST	Lyophilized recombinant human TNFRSF12A/TWEAKR standard	10ng/tube×2
EK1753-DA	Biotinylated anti- human TNFRSF12A/TWEAKR antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	47	209	855	58	247	903
Standard deviation	2.21	11.5	50.45	3.71	16.55	67.8
CV(%)	4.7	5.5	5.9	6.4	6.7	7.5

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.024	0.195	0.326	0.544	1.077	1.689	2.336	2.436

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human TWEAKR/TNFRSF12A ELISA Kit

EK1753

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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EK1754

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Human TWEAKR/TNFRSF12A ELISA Kit

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