产品名称
Rat PDGF-CC/PDGFC ELISA Kit
指标别名
FALLOTEIN; PDGF C; PDGFC; PDGFC receptor binding form; SCDGF; VEGF E
免疫原
Expression system for standard: E.coli; Immunogen sequence: A235-G345
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
platelet derived growth factor C
文献引用格式
Rat PDGF-CC/PDGFC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1855)
背景资料
Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. It is mapped to 2q33-q34. Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation.
试剂盒内容
Catalog number | Description | Quantity |
EK1855-CAP | 96-well plate precoated with anti- rat PDGF-CC antibody | 1 |
EK1855-ST | Lyophilized recombinant rat PDGF-CC standard | 10ng/tube×2 |
EK1855-DA | Biotinylated anti- rat PDGF-CC antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 16 | 113 | 464 | 23 | 117 | 472 |
Standard deviation | 0.64 | 5.31 | 24.13 | 1.17 | 6.90 | 33.51 |
CV(%) | 4.0% | 4.7% | 5.2% | 5.1% | 5.9% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.231 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)