产品名称
Rat Laminin EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
Laminin subunit alpha 1; Laminin; alpha 1; Lama1; Laminin subunit alpha 2; Lama2; Laminin subunit alpha 3; Lama3; LOC307582; rCG_24950; Laminin subunit alpha 4; Lama4; RGD1560062_predicted; rCG_19976; Laminin subunit alpha 5; Laminin; alpha 5; isoform CRA_a; Lama5; rCG_38528; Laminin subunit beta 1; Lamb1; Laminin subunit beta-2; Laminin chain B3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta (S-LAM beta); Lamb2; Laminin subunit beta 3; Laminin; beta 3; isoform CRA_a; Lamb3; rCG_20306; Laminin subunit gamma 1; Lamc1; Laminin subunit gamma 2; Lamc2; Laminin gamma 3 (Predicted); isoform CRA_bLamc3; Lamc3_predicted; rCG_45838
免疫原
Expression system for standard: from rat sarcoma basement membrane
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
基因名称
Lama1/a2/a3/a4/a5/b1/b2/b3/c1/c2/c3
文献引用格式
Rat Laminin EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0435)
背景资料
Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ0435-CA |
Rabbit anti- rat Laminin polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ0435-DA |
Biotinylated goat anti- rat Laminin polyclonal antibody (Detection Antibody) |
500 µl, 5 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ0435-ST |
Lyophilized recombinant rat Laminin standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.097 | 0.12 | 0.163 | 0.252 | 0.482 | 0.93 | 1.654 | 2.438 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
蛋白名全称
Laminin subunit alpha 1, Laminin subunit alpha 2, Laminin subunit alpha 3, Laminin subunit alpha-4, Laminin subunit alpha-5, Laminin subunit beta-1, Laminin subunit beta-2, Laminin subunit beta-3, Laminin subunit gamma-1, Laminin subunit gamma-2, Laminin subunit gamma-3
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
联系我们
关注我们
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
