Human ACE Quick ELISA Kit

96T/3580

ACE; ACE1; Angiotensin converting enzyme; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; MVCD3

Expression system for standard: NS0; Immunogen sequence: L30-L1261

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

0.78 ng/ml - 50 ng/ml

ACE

angiotensin I converting enzyme

P12821  

Human ACE Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0557)

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

15-25分钟

Catalog number	Description	Quantity
FEK0557-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1
FEK0557-ST	Human ACE standard	50ng/tube×2
FEK0557-A	Human ACE Antibody A	3ml
FEK0557-B	Human ACE Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1278	6839	24157	1358	6350	25528
Standard deviation	62.62	465.05	1087.06	86.57	482.6	1225.34
CV (%)	4.9%	6.8%	4.5%	6.5%	7.6%	4.8%

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.037	0.109	0.201	0.357	0.614	1.113	1.750	2.023

Store at 4℃ for 6 months.

human

酶联免疫双抗夹心法

1636  

cell culture supernatants, serum, plasma (heparin) and saliva.