产品名称
Human Angiopoietin-1/ANGPT1 Quick ELISA Kit
指标别名
AGP1; AGPT; ANG 1; ANG1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
免疫原
Expression system for standard: NS0; Immunogen sequence: H16-F498
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
文献引用格式
Human Angiopoietin-1/ANGPT1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0559)
背景资料
Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
试剂盒内容
Catalog number | Description | Quantity |
FEK0559-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 |
FEK0559-ST | Human Angiopoietin-1/ANGPT1 standard | 40ng/tube×2 |
FEK0559-A | Human Angiopoietin-1/ANGPT1 Antibody A | 3ml |
FEK0559-B | Human Angiopoietin-1/ANGPT1 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 251 | 1041 | 3816 | 180 | 1013 | 4617 |
Standard deviation | 18.9 | 32.79 | 140.81 | 13.75 | 27.76 | 211.92 |
CV(%) | 7.5% | 3.2% | 3.7% | 7.6% | 2.7% | 4.6% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.084 | 0.147 | 0.222 | 0.338 | 0.568 | 1.017 | 1.673 | 2.334 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell culture supernatants, serum, plasma(heparin, EDTA), saliva and human milk