产品名称
Human AFP Quick ELISA Kit
指标别名
AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP
免疫原
Expression system for standard: NS0; Immunogen sequence: R19-V609
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5,000 pg/ml
文献引用格式
Human AFP Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0651)
背景资料
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
试剂盒内容
Catalog number | Description | Quantity |
FEK0651-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 |
FEK0651-ST | Human AFP standard | 5 ng/tube×2 |
FEK0651-A | Human AFP Antibody A | 3ml |
FEK0651-B | Human AFP Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 98 | 643 | 2190 | 98 | 654 | 1986 |
Standard deviation | 4.56 | 47.58 | 100.74 | 5.58 | 56.24 | 129.09 |
CV (%) | 4.8% | 7.4% | 4.6% | 5.7% | 8.6% | 6.5% |
典型数据示例
Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.003 | 0.137 | 0.248 | 0.486 | 0.777 | 1.260 | 1.704 | 1.925 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)