Human c-Kit/CD117/KIT Quick ELISA Kit

96T/3580

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

Expression system for standard: NS0; Immunogen sequence: Q26-T520

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

156pg/ml-10000pg/ml

KIT

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

P10721

Human c-Kit/CD117/KIT Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0835)

SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Human KIT antibody	1
FEK0663-ST	lyophilized recombinant Human KIT standard	10ng/tube×2
FEK0663-A	Human KIT Antibody A	3ml
FEK0835-B	Human KIT Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	342	1263	4698	351	1236	4352
Standard deviation	20.18	54.31	272.48	24.92	69.22	278.53
CV (%)	5.9%	4.3%	5.8%	7.1%	5.6%	6.4%

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.078	0.277	0.441	0.750	1.230	1.889	2.245	2.468

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)