Human ADAM12 Quick ELISA Kit

96T/3580

ADAM 12; ADAM12; MCMP; MCMPMltna; Meltrin alpha; MLTN; MLTNA

Expression system for standard: CHO; Immunogen sequence: R29-S513

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

156 pg/ml - 10,000 pg/ml

ADAM12

Disintegrin and metalloproteinase domain-containing protein 12

O43184  

Human ADAM12 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0934)

ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

15-25分钟

Catalog number	Description	Quantity
FEK0934-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1
FEK0934-ST	Human ADAM12 standard	10ng/tube×2
FEK0934-A	Human ADAM12 Antibody A	3ml
FEK0934-B	Human ADAM12 Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	285	1490	4755	259	1498	4763
Standard deviation	16.81	75.4%9	256.77	19.68	98.86	338.17
CV (%)	5.4%%	7.1%%	5.4%	7.6%	6.6%	7.1%

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.073	0.098	0.175	0.307	0.560	1.043	1.789

Store at 4℃ for 6 months.

human

酶联免疫双抗夹心法

8038  

cell culture supernatants, serum, plasma (heparin) and urine.