产品名称
Human ADAM15 Quick ELISA Kit
指标别名
ADAM 15; ADAM15; MDC 15; MDC15; Metargidin
免疫原
Expression system for standard: NS0; Immunogen sequence: D207-T696
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4,000 pg/ml
基因名全称
Disintegrin and metalloproteinase domain-containing protein 15
文献引用格式
Human ADAM15 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1484)
背景资料
Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK1484-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 |
| FEK1484-ST | Human ADAM15 standard | 10 ng/tube×2 |
| FEK1484-A | Human ADAM15 Antibody A | 3ml |
| FEK1484-B | Human ADAM15 Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 112 | 674 | 1454 | 110 | 704 | 1511 |
| Standard deviation | 5.04 | 53.24 | 59.61 | 7.04 | 64.06 | 64.97 |
| CV (%) | 4.5% | 7.9% | 4.1% | 6.4% | 9.1% | 4.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.061 | 0.083 | 0.109 | 0.185 | 0.404 | 0.664 | 1.373 | 2.079 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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