产品名称
Human ANGPTL2 Quick ELISA Kit
指标别名
angiopoietin like 2; Angiopoietin like protein 2; Angiopoietin related protein 2; ANGPTL2; ARP2; HARP
免疫原
Expression system for standard: NS0; Immunogen sequence: Q23-H493
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20,000 pg/ml
基因名全称
angiopoietin like 2
文献引用格式
Human ANGPTL2 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK2039)
背景资料
Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
试剂盒内容
Catalog number | Description | Quantity |
FEK2039-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 |
FEK2039-ST | Human ANGPTL2 standard | 20 ng/tube×2 |
FEK2039-A | Human ANGPTL2 Antibody A | 3ml |
FEK2039-B | Human ANGPTL2 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 590 | 1670 | 7933 | 594 | 1958 | 8471 |
Standard deviation | 30.68 | 71.81 | 325.25 | 39.20 | 113.56 | 559.09 |
CV (%) | 5.2% | 4.3% | 4.1% | 6.6% | 5.8% | 6.6% |
典型数据示例
Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.036 | 0.089 | 0.122 | 0.214 | 0.404 | 0.712 | 1.255 | 1.968 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).