产品名称
Human GM-CSF High Sensitivity ELISA Kit PicoKine®
指标别名
Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A18-E144
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Granulocyte-macrophage colony-stimulating factor
文献引用格式
Human GM-CSF High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0364)
背景资料
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
试剂盒内容
Catalog number | Description | Quantity |
---|
HSEK0364-BV-CAP | Anti-Human GM-CSF High Sensitivity Pre-coated 96-well strip microplate | 1 |
HSEK0364-BV-ST | Human GM-CSF High Sensitivity Standard | 10ng/tube×2 |
HSEK0364-BV-DA | Human GM-CSF High Sensitivity Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 2 | 9 | 30 | 2 | 9 | 36 |
Standard deviation | 0.06 | 0.31 | 2.16 | 0.14 | 0.33 | 2.04 |
CV (%) | 3.2% | 3.5% | 7.2% | 6.8% | 3.7% | 5.7% |
典型数据示例
Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.034 | 0.111 | 0.171 | 0.298 | 0.520 | 0.999 | 1.690 | 2.736 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).