Human GM-CSF High Sensitivity ELISA Kit PicoKine®

96T|2880

Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF

Expression system for standard: E.coli; Immunogen sequence: A18-E144

There is no detectable cross-reactivity with other relevant proteins.

<0.1 pg/ml

1.56-100 pg/ml

CSF2

Granulocyte-macrophage colony-stimulating factor

P04141  

Human GM-CSF High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0364)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Catalog number	Description	Quantity
HSEK0364-BV-CAP	Anti-Human GM-CSF High Sensitivity Pre-coated 96-well strip microplate	1
HSEK0364-BV-ST	Human GM-CSF High Sensitivity Standard	10ng/tube×2
HSEK0364-BV-DA	Human GM-CSF High Sensitivity Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2	9	30	2	9	36
Standard deviation	0.06	0.31	2.16	0.14	0.33	2.04
CV (%)	3.2%	3.5%	7.2%	6.8%	3.7%	5.7%

Concentration (pg/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.034	0.111	0.171	0.298	0.520	0.999	1.690	2.736

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

1437  

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

3.5h