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Human GM-CSF High Sensitivity ELISA Kit PicoKine®

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HSEK0364

  • 96T ¥2880
  • 货期: 现货
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产品名称
Human GM-CSF High Sensitivity ELISA Kit PicoKine®
规格/价格
96T|2880
指标别名
Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A18-E144
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<0.1 pg/ml
检测范围
1.56-100 pg/ml
基因名称
CSF2
基因名全称
Granulocyte-macrophage colony-stimulating factor
Uniprot ID
P04141  
文献引用格式
Human GM-CSF High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0364)
背景资料
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
试剂盒内容
Catalog numberDescriptionQuantity
HSEK0364-BV-CAPAnti-Human GM-CSF High Sensitivity Pre-coated 96-well strip microplate1
HSEK0364-BV-STHuman GM-CSF High Sensitivity Standard10ng/tube×2
HSEK0364-BV-DAHuman GM-CSF High Sensitivity Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)29302936
Standard deviation0.060.312.160.140.332.04
CV (%)3.2%3.5%7.2%6.8%3.7%5.7%
典型数据示例
Concentration (pg/ml)01.563.126.2512.52550100
O.D.0.0340.1110.1710.2980.5200.9991.6902.736
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
Gene ID
1437  
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
实验时长
3.5h

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