产品名称
Mouse GM-CSF/CSF2 High Sensitivity ELISA Kit
指标别名
Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim
免疫原
Expression system for standard: E.coli; Immunogen sequence: A18-K141
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
1.56 pg/ml - 100 pg/ml
基因名全称
colony stimulating factor 2 (granulocyte-macrophage)
文献引用格式
Mouse GM-CSF/CSF2 High Sensitivity ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#HSEK0365)
背景资料
Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; liver; lung; and trophectoderm. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| HSEK0365-CAP | Anti-Human G-CSF/CSF3 High Sensitivity Pre-coated 96-well strip microplate | 1 |
| HSEK0365-ST | Human G-CSF/CSF3 High Sensitivity Standard | 10ng/tube×2 |
| HSEK0365-DA | Human G-CSF/CSF3 High Sensitivity Biotinylated antibody (100x) | 100µl |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2.78 | 8.67 | 45.71 | 2.25 | 7.03 | 47.24 |
| Standard deviation | 0.0734 | 0.458 | 3.542 | 0.07 | 0.29 | 2.182 |
| CV (%) | 2.6% | 5.3% | 7.8% | 3.1% | 4.1% | 4.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.016 | 0.118 | 0.232 | 0.425 | 0.797 | 1.329 | 1.883 | 2.222 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).