产品名称
Mouse IFN Gamma High Sensitivity ELISA Kit PicoKine®
指标别名
Interferon gamma; IFN-gamma; Ifng
免疫原
Expression system for standard: E.coli; Immunogen sequence: H23-C155
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IFN Gamma High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0375)
背景资料
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
试剂盒内容
Catalog number | Description | Quantity |
---|
HSEK0375-BV-CAP | Anti-Mouse IFN Gamma High Sensitivity Pre-coated 96-well strip microplate | 1 |
HSEK0375-BV-ST | Mouse IFN Gamma High Sensitivity Standard | 10ng/tube×2 |
HSEK0375-BV-DA | Mouse IFN Gamma High Sensitivity Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 4 | 21 | 87 | 5 | 20 | 58 |
Standard deviation | 0.31 | 1.55 | 3.10 | 0.35 | 1.35 | 1.62 |
CV (%) | 7.8% | 7.4% | 3.6% | 6.9% | 6.8% | 2.8% |
典型数据示例
Concentration (pg/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.058 | 0.101 | 0.136 | 0.218 | 0.351 | 0.602 | 1.131 | 1.913 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants and serum.