产品名称
Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®
指标别名
Interleukin-1 beta; IL-1 beta; IL1B
免疫原
Expression system for standard: E.coli; Immunogen sequence: V118-S269
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0394)
背景资料
Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| HSEK0394-BV-CAP | Anti-Mouse IL-1 Beta High Sensitivity Pre-coated 96-well strip microplate | 1 |
| HSEK0394-BV-ST | Mouse IL-1 Beta High Sensitivity Standard | 10ng/tube×2 |
| HSEK0394-BV-DA | Mouse IL-1 Beta High Sensitivity Biotinylated antibody (100x) | 100µl |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3 | 9 | 44 | 2 | 9 | 25 |
| Standard deviation | 0.18 | 0.50 | 1.17 | 0.12 | 0.55 | 1.27 |
| CV (%) | 6.1% | 5.5% | 2.7% | 6.0% | 6.1% | 5.1% |
典型数据示例
| Concentration (pg/ml) | 0 | >1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.071 | 0.136 | 0.175 | 0.271 | 0.428 | 0.752 | 1.279 | 2.047 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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