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Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®

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HSEK0394

  • 96T ¥2880
  • 货期: 现货
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产品名称
Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®
规格/价格
96T|2880
指标别名
Interleukin-1 beta; IL-1 beta; IL1B
免疫原
Expression system for standard: E.coli; Immunogen sequence: V118-S269
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<0.23 pg/ml
检测范围
1.56-100 pg/ml
基因名称
IL1B
基因名全称
Interleukin-1 beta
Uniprot ID
P10749  
文献引用格式
Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0394)
背景资料
Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
试剂盒内容
Catalog numberDescriptionQuantity
HSEK0394-BV-CAPAnti-Mouse IL-1 Beta High Sensitivity Pre-coated 96-well strip microplate1
HSEK0394-BV-STMouse IL-1 Beta High Sensitivity Standard10ng/tube×2
HSEK0394-BV-DAMouse IL-1 Beta High Sensitivity Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)39442925
Standard deviation0.180.501.170.120.551.27
CV (%)6.1%5.5%2.7%6.0%6.1%5.1%
典型数据示例
Concentration (pg/ml)0>1.563.126.2512.52550100
O.D.0.0710.1360.1750.2710.4280.7521.2792.047
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
Gene ID
16176  
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
实验时长
3.5h

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