产品名称
Mouse IL-2 High Sensitivity ELISA Kit PicoKine®
指标别名
Interleukin-2; IL-2; T-cell growth factor; TCGF; Il2; Il-2
免疫原
Expression system for standard: E.coli; Immunogen sequence: A21-Q169
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL-2 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0398)
背景资料
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
HSEK0398-BV-CAP | Anti-Mouse IL-2 High Sensitivity Pre-coated 96-well strip microplate | 1 |
HSEK0398-BV-ST | Mouse IL-2 High Sensitivity Standard | 10ng/tube×2 |
HSEK0398-BV-DA | Mouse IL-2 High Sensitivity Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1 | 2 | 8 | 1 | 2 | 5 |
Standard deviation | 0.06 | 0.09 | 0.55 | 0.02 | 0.1 | 0.22 |
CV (%) | 6.2% | 4.4% | 6.9% | 2.5% | 4.9% | 4.3% |
典型数据示例
Concentration (pg/ml) | 0 | 0.31 | 0.62 | 1.25/td> | 2.5 | 5 | 10 | 20 |
O.D. | 0.053 | 0.126 | 0.188 | 0.301 | 0.536 | 0.973 | 1.747 | 2.880 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).