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Human IL-6 High Sensitivity ELISA Kit PicoKine®

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HSEK0410

  • 96T ¥2880
  • 货期: 现货
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产品名称
Human IL-6 High Sensitivity ELISA Kit PicoKine®
规格/价格
96T|2880
指标别名
Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2
免疫原
Expression system for standard: E.coli; Immunogen sequence: P29-M212
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<0.108 pg/ml
检测范围
0.78-50 pg/ml
基因名称
IL6
基因名全称
interleukin 6
Uniprot ID
P05231  
文献引用格式
Human IL-6 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0410)
背景资料
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
试剂盒内容
Catalog numberDescriptionQuantity
HSEK0410-BV-CAPAnti-Human IL-6 High Sensitivity Pre-coated 96-well strip microplate1
HSEK0410-BV-STHuman IL-6 High Sensitivity Standard10ng/tube×2
HSEK0410-BV-DAHuman IL-6 High Sensitivity Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15161417
Standard deviation0.070.140.650.060.131.03
CV (%)7.3%2.9%4.1%5.6%3.4%6.1%
典型数据示例
Concentration (pg/ml)00.781.563.126.2412.52550
O.D.0.0370.1250.1730.2820.4520.8471.4022.167
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
Gene ID
3569  
样本类型
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
实验时长
3.5h

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