产品名称
Mouse IL-6 High Sensitivity ELISA Kit PicoKine®
指标别名
Interleukin 6 ; Interleukin 6, isoform CRA_b ; IL6 ; mCG_11634
免疫原
Expression system for standard: E.coli; Immunogen sequence: F25-T211
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL-6 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0411)
背景资料
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
HSEK0411-BV-CAP | Anti-Mouse IL-6 High Sensitivity Pre-coated 96-well strip microplate | 1 |
HSEK0411-BV-ST | Mouse IL-6 High Sensitivity Standard | 10ng/tube×2 |
HSEK0411-BV-DA | Mouse IL-6 High Sensitivity Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 3 | 9 | 29 | 2 | 10 | 27 |
Standard deviation | 0.23 | 0.58 | 1.27 | 0.05 | 0.65 | 1.59 |
CV (%) | 7.7% | 6.5% | 4.4% | 2.6% | 6.5% | 5.9% |
典型数据示例
Concentration (pg/ml) | 0 | >1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.030 | 0.117 | 0.172 | 0.315 | 0.506 | 0.939 | 1.775 | 2.481 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).