产品名称
Human IL-8 High Sensitivity ELISA Kit PicoKine®
指标别名
Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8)77; GCP/IL-8 protein II; IL-8 (1-77); IL8/NAP1 form II; MDNCF-b; IL-8 (5-77); IL-8 (6-77); (Ser-IL-8)72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77); GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77); GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77); GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8
免疫原
Expression system for standard: E.coli; Immunogen sequence: S28-S99
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human IL-8 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0413)
背景资料
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| HSEK0413-BV-CAP | Anti-Human IL-8 High Sensitivity Pre-coated 96-well strip microplate | 1 |
| HSEK0413-BV-ST | Human IL-8 High Sensitivity Standard | 10ng/tube×2 |
| HSEK0413-BV-DA | Human IL-8 High Sensitivity Biotinylated antibody (100x) | 100µl |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3 | 10 | 30 | 3 | 7 | 29 |
| Standard deviation | 0.08 | 0.37 | 0.94 | 0.08 | 0.17 | 0.98 |
| CV (%) | 2.5% | 3.7% | 3.1% | 2.8% | 2.4% | 3.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.017 | 0.066 | 0.105 | 0.166 | 0.298 | 0.592 | 1.082 | 1.945 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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