产品名称
Human IL10 High Sensitivity ELISA Kit
指标别名
CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
免疫原
Expression system for standard: E.coli; Immunogen sequence: S19-N178
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 pg/ml - 50 pg/ml
文献引用格式
Human IL10 High Sensitivity ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#HSEK0416)
背景资料
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| HSEK0416-CAP | Anti-Human IL10 High Sensitivity Pre-coated 96-well strip microplate | 1 |
| HSEK0416-ST | Human IL10 High Sensitivity Standard | 10ng/tube×2 |
| HSEK0416-DA | Human IL10 High Sensitivity Biotinylated antibody (100x) | 100µl |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1.19 | 3.45 | 19.69 | 1.22 | 3.53 | 13.82 |
| Standard deviation | 0.082 | 0.094 | 1.365 | 0.036 | 0.132 | 0.843 |
| CV (%) | 6.9% | 2.7% | 6.9% | 2.9% | 3.7% | 6.1% |
典型数据示例
| Concentration (pg/ml) | 0 | 0.78 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.029 | 0.12 | 0.21 | 0.383 | 0.725 | 1.235 | 2.044 | 3.25 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).