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Mouse IL-17 High Sensitivity ELISA Kit PicoKine®

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HSEK0431

  • 96T ¥2880
  • 货期: 现货
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产品名称
Mouse IL-17 High Sensitivity ELISA Kit PicoKine®
规格/价格
96T|2880
指标别名
Interleukin 17A ; Il17a ; Il17 ; mCG_7914
免疫原
Expression system for standard: E.coli; Immunogen sequence: T22-A158
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<0.03 pg/ml
检测范围
0.78-50 pg/ml
基因名称
IL17A
基因名全称
interleukin 17A
Uniprot ID
Q544E6  
文献引用格式
Mouse IL-17 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0431)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
试剂盒内容
Catalog numberDescriptionQuantity
HSEK0431-BV-CAPAnti-Mouse IL-17 High Sensitivity Pre-coated 96-well strip microplate1
HSEK0431-BV-STMouse IL-17 High Sensitivity Standard10ng/tube×2
HSEK0431-BV-DAMouse IL-17 High Sensitivity Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14181420
Standard deviation0.020.301.310.070.091.10
CV (%)2.1%7.4%7.3%7.2%2.4%5.5%
典型数据示例
Concentration (pg/ml)00.781.563.126.2412.52550
O.D.0.0470.1080.1650.2810.4670.7951.4712.356
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
Gene ID
16171  
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
实验时长
3.5h

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