产品名称
Mouse IL-17 High Sensitivity ELISA Kit PicoKine®
指标别名
Interleukin 17A ; Il17a ; Il17 ; mCG_7914
免疫原
Expression system for standard: E.coli; Immunogen sequence: T22-A158
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL-17 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0431)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
HSEK0431-BV-CAP | Anti-Mouse IL-17 High Sensitivity Pre-coated 96-well strip microplate | 1 |
HSEK0431-BV-ST | Mouse IL-17 High Sensitivity Standard | 10ng/tube×2 |
HSEK0431-BV-DA | Mouse IL-17 High Sensitivity Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1 | 4 | 18 | 1 | 4 | 20 |
Standard deviation | 0.02 | 0.30 | 1.31 | 0.07 | 0.09 | 1.10 |
CV (%) | 2.1% | 7.4% | 7.3% | 7.2% | 2.4% | 5.5% |
典型数据示例
Concentration (pg/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.24 | 12.5 | 25 | 50 |
O.D. | 0.047 | 0.108 | 0.165 | 0.281 | 0.467 | 0.795 | 1.471 | 2.356 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).