Rat TNF Alpha/TNFA High Sensitivity ELISA Kit

96T|2880

Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor

Expression system for standard: E.coli; Immunogen sequence: L80-L235

There is no detectable cross-reactivity with other relevant proteins.

0.006pg/ml

1.56 pg/ml - 100 pg/ml

TNF

tumor necrosis factor

P16599  

Rat TNF Alpha/TNFA High Sensitivity ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#HSEK0526)

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including negative regulation of transport; positive regulation of cell communication; and positive regulation of macromolecule biosynthetic process. Acts upstream of or within positive regulation of interleukin-6 production. Located in external side of plasma membrane; extracellular space; and neuronal cell body. Is active in extracellular region. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); congestive heart failure (multiple); liver disease (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); liver disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Catalog number	Description	Quantity
HSEK0526-CAP	Anti-Rat TNF Alpha/TNFA High Sensitivity Pre-coated 96-well strip microplate	1
HSEK0526-ST	Rat TNF Alpha/TNFA High Sensitivity Standard	10ng/tube×2
HSEK0526-DA	Rat TNF Alpha/TNFA High Sensitivity Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2.97	11.67	40.62	2.79	11.85	32.84
Standard deviation	0.059	0.447	2.019	0.136	0.558	1.593
CV (%)	2.0%	3.8%	5.0%	4.9%	4.7%	4.9%

Concentration (pg/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.041	0.089	0.125	0.18	0.274	0.475	0.958	1.883

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

24835  

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).