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Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®

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HSEK0527

  • 96T ¥2880
  • 货期: 现货
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产品名称
Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®
规格/价格
96T|2880
指标别名
Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2
免疫原
Expression system for standard: E.coli; Immunogen sequence: L80-L235
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<0.006 pg/ml
检测范围
1.56-100 pg/ml
基因名称
TNF
基因名全称
Tumor necrosis factor
Uniprot ID
P06804  
文献引用格式
Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0527)
背景资料
Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
试剂盒内容
Catalog numberDescriptionQuantity
HSEK0527-BV-CAPAnti-Mouse TNF Alpha High Sensitivity Pre-coated 96-well strip microplate1
HSEK0527-BV-STMouse TNF Alpha High Sensitivity Standard10ng/tube×2
HSEK0527-BV-DAMouse TNF Alpha High Sensitivity Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2104231034
Standard deviation0.100.441.970.210.401.28
CV (%)5.0%4.4%4.7%7.0%4.0%3.8%
典型数据示例
Concentration (pg/ml)01.563.126.2512.52550100
O.D.0.0740.1560.2150.3490.6181.0261.6762.338
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
Gene ID
21926  
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
实验时长
3.5h

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