Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®

96T|2880

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

Expression system for standard: E.coli; Immunogen sequence: L80-L235

There is no detectable cross-reactivity with other relevant proteins.

<0.006 pg/ml

1.56-100 pg/ml

TNF

Tumor necrosis factor

P06804  

Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Wuhan, China. Catalog#HSEK0527)

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Catalog number	Description	Quantity
HSEK0527-BV-CAP	Anti-Mouse TNF Alpha High Sensitivity Pre-coated 96-well strip microplate	1
HSEK0527-BV-ST	Mouse TNF Alpha High Sensitivity Standard	10ng/tube×2
HSEK0527-BV-DA	Mouse TNF Alpha High Sensitivity Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2	10	42	3	10	34
Standard deviation	0.10	0.44	1.97	0.21	0.40	1.28
CV (%)	5.0%	4.4%	4.7%	7.0%	4.0%	3.8%

Concentration (pg/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.074	0.156	0.215	0.349	0.618	1.026	1.676	2.338

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

21926  

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

3.5h