Anti-IDH1 Antibody (Clone#OTI3G9)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00129-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-IDH1 antibody (M00129-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH1 antigen affinity purified monoclonal antibody (M00129-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH1 at approximately 47 kDa. The expected band size for IDH1 is at 47 kDa.
all(2)
IHC analysis of IDH1 using anti-IDH1 antibody (M00129-3) .
IDH1 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with mouse anti-IDH1 Antibody (M00129-3) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-IDH1 Antibody (Clone#OTI3G9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

IDCD; IDH; IDH1; IDP; IDPC; NADP(+) specific ICDH; Oxalosuccinate decarboxylase; PICD

产品类型

Monoclonal

检验物种

human, mouse, rat, dog

应用范围

WB, IHC

基因名称

IDH1

克隆号

OTI3G9

宿主

Mouse

抗体亚型

IgG1

免疫原

Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH

计算分子量

47 kDa

实际分子量

47 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Isocitrate dehydrogenase 1 (NADP+), soluble is an enzyme that in humans is encoded by the IDH1 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Uniprot ID

O75874

文献引用格式

IDH1 Antibody (Clone#OTI3G9) (Boster Biological Technology, Wuhan, China. Catalog#M00129-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Isocitrate dehydrogenase [NADP] cytoplasmic|IDH|EC 1.1.1.42|Cytosolic NADP-isocitrate dehydrogenase|IDP|NADP(+-specific ICDH|Oxalosuccinate decarboxylase

蛋白名全称

Isocitrate dehydrogenase [NADP] cytoplasmic

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