Immunohistochemistry (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human tonsil cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
IF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of Jurkat cells using anti-Ki67/MKI67 antibody (M00254-8).
Overlay histogram showing Jurkat cells stained with M00254-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human tonsil cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
IF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-8).
Ki67/MKI67 was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of Jurkat cells using anti-Ki67/MKI67 antibody (M00254-8).
Overlay histogram showing Jurkat cells stained with M00254-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67/MKI67 Antibody (M00254-8) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.