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Anti-IL-17/IL17A Antibody

Mouse monoclonal antibody

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筛选器: All IHC

M00421-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • IHC analysis of IL17A using anti-IL17A antibody (M00421-2).
    IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4~C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37~C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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产品名称
Anti-IL-17/IL17A Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A
产品类型
Monoclonal
检验物种
mouse
应用范围
IHC
基因名称
Il17a
克隆号
AFIF-9
宿主
Mouse
抗体亚型
IgG1, κ
免疫原
Mouse IL-17A cross-linked to OVA
成分
200 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
200 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Uniprot ID
Q62386  
文献引用格式
IL-17/IL17A Antibody (Boster Biological Technology, Wuhan, China. Catalog#M00421-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P) .
基因名全称
interleukin 17A
蛋白名全称
Interleukin-17A
推荐稀释比
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-IL-17/IL17A Antibody

    筛选器: All IHC

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