Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of IL17A using anti-IL17A antibody (M00421-2).
IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4~C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37~C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IHC analysis of IL17A using anti-IL17A antibody (M00421-2).
IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4~C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37~C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.