| Western blot (WB): | 1:2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
| Flow cytometry (FCM): | 1:100 |

Flow cytometric Analysis of Hela cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunofluorescent staining of HeLa cells using anti-FGF21 mouse monoclonal antibody.

Flow cytometric Analysis of Jurkat cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.

Flow cytometric Analysis of Hela cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunofluorescent staining of HeLa cells using anti-FGF21 mouse monoclonal antibody.

Flow cytometric Analysis of Jurkat cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.



