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Anti-CD55 Antibody (Clone#5B9E1)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF IF

M00910-4

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).

    all(8)
  • Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human placenta tissue lysates,
    Lane 2: HELA whole cell lysates,
    Lane 3: PC-3 whole cell lysates,
    Lane 4: CACO-2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.

    all(8)
  • Figure 2. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 3. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 4. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 5. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 6. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 7. IF analysis of CD55 using anti-CD55 antibody (M00910-4).
    CD55 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(8)
  • Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).

    all(8)
  • Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human placenta tissue lysates,
    Lane 2: HELA whole cell lysates,
    Lane 3: PC-3 whole cell lysates,
    Lane 4: CACO-2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.

    all(8)

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产品简介>

产品名称
Anti-CD55 Antibody (Clone#5B9E1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD55; CR; CROM; DAF; TC
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, IF
基因名称
CD55
克隆号
5B9E1
宿主
Mouse
抗体亚型
IgG1
免疫原
E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
计算分子量
41 kDa
实际分子量
70-75 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Uniprot ID
P08174  
文献引用格式
CD55 Antibody (Clone#5B9E1) (Boster Biological Technology, Wuhan, China. Catalog#M00910-4)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
蛋白名全称
Complement decay-accelerating factor
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence(ICC/IF):1:50-400
Immunofluorescence (IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-CD55 Antibody (Clone#5B9E1)

    筛选器: All WB IHC ICC/IF IF

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