Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(8)
Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: HELA whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.
Figure 2. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(8) Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: HELA whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: HELA whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.
Figure 2. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: HELA whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (M00910-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.
Figure 2. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of CD55 using anti-CD55 antibody (M00910-4).
CD55 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-CD55 Antibody (M00910-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. IF analysis using anti- CD55 Antibody (M00910-4). detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
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