Anti-COL4A1 Antibody (Clone#3G3)

Mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All IHC WB IF

M01411

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

IHC analysis of COL4A1 using anti-COL4A1 antibody (M01411).
COL4A1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-COL4A1 Antibody (M01411) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of COL4A1 using anti-COL4A1 antibody (M01411).
COL4A1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-COL4A1 Antibody (M01411) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Western blot analysis of COL4A1 using anti-COL4A1 antibody (M01411). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-COL4A1 antigen affinity purified monoclonal antibody (M01411) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COL4A1 at approximately 220 kDa. The expected band size for COL4A1 is at 161 kDa.
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-COL4A1 Antibody (Clone#3G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

collagen type IV alpha 1 chain; BSVD; BSVD1s; PADMAL; RATOR; COL4A1

产品类型

Monoclonal

检验物种

human

应用范围

IHC, WB, IF

基因名称

COL4A1

克隆号

3G3

宿主

Mouse

抗体亚型

IgG1

免疫原

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

计算分子量

161 kDa

实际分子量

220 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Uniprot ID

P02462

RRID

AB_3081839

文献引用格式

COL4A1 Antibody (Clone#3G3) (Boster Biological Technology, Wuhan, China. Catalog#M01411)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除